June 14, 2016
Raman Microscopy Workshop – July 19 – July 21, 2016
The CIF is hosting a workshop on high performance confocal Raman microscopy and imaging on Tuesday, July 19 through Thursday, July 21. The workshop is open to all CSU faculty, staff and students at no cost. Please register in advance by sending an email to firstname.lastname@example.org.
For more information, see flyer
June 10, 2015
Surface Plasmon Resonance – SPR
The CIF inherited a GE Biacore T200 instrument. This instrument brings a new technology called Surface Plasmon Resonance, or SPR, to the CIF spectroscopy laboratory that will serve several of our chemical biology faculty as well as faculty at other departments (microbiology, engineering, biochemistry) on the main campus.
SPR is a phenomenon that allows real-time, label-free detection of biomolecular interactions. Applications of SPR include biotherapeutic and drug discovery research, as well as protein activity and stability analysis in biopharmaceutical production. In the T200 instrument, target molecules, most frequently proteins, are immobilized on a prepared gold sensor surface and a sample containing a potential interacting partner in solution is injected over the surface through a series of flow cells. During the course of the interaction, polarized light is directed toward the sensor surface and the angle of minimum intensity reflected light is detected. This angle changes as molecules bind and dissociate and the interaction profile is thus recorded in real time. As light does not penetrate the sample, interactions can be followed in colored, turbid, or opaque samples. No labels are required and since detection is based on changes in plasmon resonance, it is nearly instantaneous. For more information on this instrument and its capabilities, please contact Karolien Denef, or visit: https://www.biacore.com/lifesciences/introduction
November 21, 2014
New Sample Submission Form for Mass Spec Facility
To all users of the Mass Spec Facility:
Please start using our new Sample Submission Form for any samples submitted for analysis by MS. We will no longer accept older forms after December 5. A digital version of this form is available on the MS facility’s web page
as well as here.
October 21, 2014
CIF Summer School 2015 – Electron Diffraction Methods for Materials Analysis
We are excited to announce the first short-course of our annual Summer School. This 3-day workshop (May 19-21, 2015) will introduce students to different electron diffraction methods for microstructural crystallographic characterization of materials in SEM and TEM.
Topics will include: conventional diffraction modes in TEM; precession electron diffraction; and electron backscatter diffraction in SEM.
The course will be open to students, researchers and professionals in the field. Registration will open January 15, 2015. Please visit our Summer School page for more information.
September 16, 2014
New Instruments and Upgrades at the CIF
Below is a short description of our newest instrument additions and upgrades. Please have your students sign up for training using our online form if they are interested in using any of these instruments. Please contact Karolien Denef or Chris Rithner with any questions.
Horiba FluoroLog-3 Spectrofluorometer (NEW!)
High sensitive, modular spectrofluorometer for fluorescence measurements in the 240nm to 850nm spectral range.
Malvern Zetasizer Nano ZS (NEW!)
For measurements of particle size (0.6nm – 6um) (using Dynamic Light Scattering), zeta potential and molecular weight of particles and molecules in liquids.
Quantum Design PPMS (NEW!)
Physical Property Measurement System for measuring magnetic, thermal and electrical properties of materials.
Olympus IX73 Fluorescence Microscope (NEW!)
For high-quality imaging of bacterial and mammalian cells in the Cell Culture Facility.
Currently equipped with filter cubes for DAPI, RFP and GFP, but can accommodate more cubes based on specific needs (up to 10).
Access to this scope requires specific biosafety training and documentation due to location in a regulated BSL2-facility.
Mercury Plus NMR console (JS300 lab)
Upgrade to the old console and data system.
Software package nearly identical with that used on the rest of the CIF NMRs, complete with an operator login that is unique for each user, more robust file system, networking abilities, etc..
400NMR console and magnet
To replace quenched 300MHz magnet. Will also have limited solid-state NMR capabilities (under development).
Thermo LTQ Mass Spec
Donation from the PMF facility, replacing our old Thermo LCQDuo Mass Spec. Offers significant improvement in terms of sensitivity and scan rate.
Currently only equipped with ESI source. Likely future additions include an APCI source and protein deconvolution software.
August 02, 2014
JS300 – 3rd Floor – NMR Users / UPDATE
The data system upgrade to the JS-300 3rd Floor NMR has gone mostly well and so the instrument may not be used – cautiously. Please let me, cdd, know if you encounter unusual problems.
The interface is different, of course, and you can only do PROTON NMR on the instrument for the time being. There is a problem with the amplifier module that I’m sorting out but PROTON should work fairly well. The S/N will be a bit low until the amps are repaired. The instrument spins samples nicely and is pretty well shimmed up.
The software is locked down. You should manually lock and shim, recall the “default shims” from the shim panel if you have troubles, then start from there.
CDCl3 Z0 is about -277 or so.
LOCKPHASE is about 25 or so (leave this alone, please, unless someone else plays with it)
DO NOT run autoshim, findz0, AutoLock, gradients shimming or anything like that. The instrument is not equipped and is not configured for these things (and they will SLOW YOU DOWN anyway).
Once your data is collected then wait for the Study Queue to update, open your Study (double click, drag, etc.) and check your spectrum. The data is mailed to you as usual.
July 18, 2014
Light Scattering Workshop (Monday, July 21)
For more information, please download the full agenda here.
July 16, 2014
JS300 – 3rd Floor – NMR Users
There is an upgrade to the JS300 NMR data system scheduled to begin August 1; that’s a Friday. Depending on how things go then the upgrade may take the weekend to accomplish – through August 4. The JS300 NMR will be unavailable during this time so you will want to make alternative plans. We don’t expect any major problems since we have tested the software on the complete hardware package, console, etc. that is must control (but not with the magnet, obviously, or the preamp module yet), however, until we “go live” it is difficult to anticipate all circumstance. In the event that there is some major problem with the upgrade then we will back it out and restore the original functionality. In any case, as soon as the instrument is ready then it will go live. This event will be reported on the CIF News website but will also be obvious to those dwelling on the 3rd floor.
We are doing this because the old console and data system are *way* past their prime and out of step with other elements in our lab. When finished, the software package will be nearly identical with that used on the rest of the CIF NMRs, complete with an operator login that is unique for each user (identical to those used downstairs), more robust file system, networking abilities, etc.. The most obvious differences will be that the console hardware package is more limited on this instrument compared with our other NMRs since the JS300 is still dedicated to rapid collection of proton NMRs in a user-directed, high throughput laboratory. There will be no automation via sample changer (other than yourselves), you will be able to lock and shim manually if you like – or not at all.
Those of you who have not become acquainted with the new Agilent NMR software, VnmrJ, and how it interacts with you and the NMR console may want to consider priming yourselves. If you have not registered for basic NMR training yet, then you should consider doing that here: http://CIF.Colostate.EDU/cif-user-registration/ and then explore the rest of the “CIF” site while you are at it. This will give you a coveted “NMR login” (once you receive basic training) and access to the entire fleet of NMR spectrometers, besides the JS300.
May 08, 2014
Summer School Keynote – Dr. Thomas Patapoff
Friday June 6, 2014 – Chemistry A101 (open to the general public)
Use of Multiple Analytical Techniques to Better Understand the Origins of High Viscosity Biopharmaceutical Products
For abstract & bio, click here.
Thomas W. Patapoff, Ph.D.
Early Stage Pharmaceutical Development
A Member of the Roche Group
April 21, 2014
TEM Open House
Announcement by Ellen R. Fisher, Professor and Chair of Chemistry Department, CSU:
It is my pleasure to invite you to our upcoming open house to celebrate the much anticipated official launch of our new High Resolution Transmission Electron Microscope. I am truly excited about our expanded capabilities, as well as the newly renovated CIF electron microscopy center and our dedicated EM staff. There is no doubt this instrument will truly transform materials research at CSU. I hope you can drop by and see first-hand what all the buzz is about!