Mass Spectrometry Laboratory

LC-TOF/MS

The CIF has a eight mass spectrometers with a variety of ionization techniques available for molecular mass measurements of small molecules, biomolecules (e.g. proteins, polysaccharides) and nano materials (e.g. gold nano clusters). The facility offers a wide range of services, including (1) accurate mass analysis for generating precise chemical formulae and MS/MS for further structural elucidation; (2) targeted quantitative analysis by triple quad MS; (3) intact protein analysis; and (4) targeted and untargeted metabolite and small molecule screening in biological samples. We also provide method development services.
Two of our instruments are open for walk-up use by trained CSU researchers and their students. One TOF LC/MS instrument is operated only by CIF staff members. All other instruments are open-access following training, reservation and project approval by CIF staff

First-time users, please register at here to sign up for training or to register as a new user.

Walk-up Instruments (Self-service)

GCMS

GC/MS: Agilent 5973N Mass Selective Detector interfaced to a 6890 gas chromatograph which is equipped with a 7683 automatic liquid sampler.  This instrument is a single quadrupole mass spectrometer utilizing an electron ionization source (EI).

 
 
 
 
LTQ LCMSn

Ion-trap LC/MS-MS: Thermo-Finnigan LTQ LC/MS-MS. This instrument is a linear ion trap mass spectrometer with electrospray ionization (ESI), interfaced to an HP 1100 high performance liquid chromatograph. Allows MSn scanning for multi-stage MS experiments to probe the structure of ions.

 
 
 
 

Quantitative Targeted Analyses (Self-service)

GC triple quad MS

GC triple quadrupole MS: GC/MSMS – Thermo Scientific TSQ 8000 Evo Triple Quadrupole GC-MS/MS, equipped with automatic liquid sampler and EI and CI sources.
 
 
 
 
 
 
UPLC triple quad MS
LC triple quadrupole MS: Waters Xevo UPLC-MS/MS triple quad with ESI ionization and UPLC

 
 
 
 
 
 

Accurate-Mass Analyses (CIF operated)

Time of Flight TOF LC-MS and Q-TOF LC-MS/MS: for accurate mass measurements with typically  less than +/-2 ppm mass measurement error. Sample introduction can be done by HPLC, UPLC, flow injection or syringe infusion.
Agilent LC-TOF-MS

  1. Agilent 6220 TOF LC/MS (“OTOF”) interfaced to an Agilent 1200 HPLC with electrospray (ESI), multi-mode (combined ESI and APCI), atmospheric pressure photoionization (APPI), and Direct Analysis in Real Time (DART) sources.
  2. Agilent 6224 TOF LC/MS (“MTOF”) interfaced to an Agilent 1260 HPLC with electrospray and multi-mode (ESI/APCI) sources.
  3. Agilent 6224 TOF LC/MS (“BTOF”) interfaced to an Agilent 1290 Infinity UHPLC with electrospray, multi-mode (ESI/APCI) sources. Located in BMAC (Foothills Campus)
  4. Agilent 6510A Q-TOF LC/MS/MS (“QTOF”) interfaced to an Agilent 1200 HPLC with electrospray, multi-mode (ESI/APCI) sources. Located in BMAC (Foothills Campus)

 

Location and Contact
CIF Mass Spectrometry Laboratory
CIF MS laboratory – Chemistry C03, CSU main campus
BMAC Satellite laboratory – RIC, Foothills Campus
 
Karolien Denef
Phone: (970) 491-3832
Email: Karolien.Denef@colostate.edu

 
 
 
 
 
 
 

Additional information on instruments, training and services

CIF Mass Spec Sample Submission Form-PDF.

CIF Mass Spec Sample Submission Form-WORD. Please use this form if you want to include a Chemdraw image of your compound structure.